The goal of this project is to determine and compare the effects of various flavorings in e-cigarettes, cigarillos, and waterpipe tobacco on toxicological and immune-inflammatory responses. Study aims are:
(1) to determine comparative in vitro toxicity of selected tobacco product flavorings using (a) the aerosol exposure system for cell-free reactive oxygen species reactivity, and (b) exposure to human lung epithelial cells via air-liquid interface and 3D culture;
(2) to determine comparative oxidative, DNA damage and immune-inflammatory responses to tobacco product flavorings in a mouse model to determine the link between flavoring toxicity and adverse respiratory health outcomes; and
(3) to identify comparative biomarkers in response to tobacco product flavorings. Assessment of toxicity within the same class of flavorings across different types of tobacco products will allow a toxicity/hazard ranking and information related to flavoring-specific adverse health outcomes.